Seventh Semester

Pokhara University

B.Sc. Biochemistry (Revised Syllabus)

Biochem 413 (Food and Nutrition Biochemistry) 

Section I

Macronutrients and Their Metabolism; Dietary Fiber; Lipids, Lipoproteins, and Cardiovascular Disease Risk; Integration and Regulation of Metabolism and the Impact of Exercise and port; Macrominerals: Calcium, Phosphorous, Phosphorus, Sodium, Potassium, and Chloride

Section II

Micro minerals: Iron, Zinc, Copper, Selenium, Chromium, Iodine, Manganese, Molybdenum, Fluorine

Section III

Ultra trace Elements: Nickel, Silicon, Vanadium, Arsenic, Boron, Cobalt

Section IV

Homeostatic Maintenance, Body fluid and electrolyte balance , Body composition and energy expenditure: Nutrition and the central nervous system, Nutrition knowledge base

Text book:

Groff and Cropper, Advanced nutrition and human metabolism, third edition, Wadsworth Publication, Thomson Learning

Clinical and Medical BioChemistry (Biochem 411)

Section I

Biochemistry & Medicine; Water & pH; Amino Acids & Peptides; Proteins: Determination of Primary Structure; Proteins: Higher Orders of Structure; Proteins:Myoglobin & Hemoglobin Proteins: Myoglobin & Hemoglobin; Enzymes: Mechanism of Action; Enzymes: Kinetics Enzymes: Regulation of Activities; Bioenergetics: The Role of ATP; Biological Oxidation; The Respiratory Chain & Oxidative Phosphorylation; Carbohydrates of Physiological Significance; Lipids of Physiologic Significance

Section II

Overview of Metabolism; The Citric Acid Cycle: The Catabolism of Acetyl-CoA; Glycolysis & the Oxidation of Pyruvate; Metabolism of Glycogen; Gluconeogenesis & Control of the Blood Glucose; The Pentose Phosphate Pathway & Other Pathways of Hexose Metabolism; Biosynthesis of Fatty Acids; Oxidation of Fatty Acids: Ketogenesis; Metabolism of Unsaturated Fatty Acids & Eicosanoids; Metabolism of Acylglycerols & Sphingolipids; Lipid Transport & Storage; Cholesterol Synthesis, Transport, & Excretion;Integration of Metabolism—the Provision of Metabolic Fuels; Biosynthesis of the Nutritionally Nonessential Amino Acids; Catabolism of Proteins & of Amino Acid Nitrogen; Catabolism of the Carbon Skeletons of Amino Acids ; Conversion of Amino Acids to Specialized Products; Porphyrins & Bile Pigments

Section III

Nucleotides; Metabolism of Purine & Pyrimidine Nucleotides; Nucleic Acid Structure & Function; DNA Organization, Replication, & Repair;RNA Synthesis, Processing, & Modification; Protein Synthesis & the Genetic Code; Regulation of Gene Expression; Molecular Genetics, Recombinant DNA, & Genomic Technology; Membranes: Structure & Function; The Diversity of the Endocrine System; Hormone Action & Signal Transduction

Section IV

Nutrition, Digestion, & Absorption; Vitamins & Minerals; Intracellular Traffic & Sorting of Proteins; Glycoproteins; The Extracellular Matrix; Muscle & the Cytoskeleton; Plasma Proteins & Immunoglobulins; Hemostasis & Thrombosis; Red & White Blood Cells Metabolism of Xenobiotics; The Human Genome Project

Text Book:

Murray RK, Granner DK, Nayes PA and Rodwell(2005) Harper's Illustrated Biochemistry,Twenty-Sixth Edition Appleton and Longe Medical Publishers and McGraw Hill Company Inc.

Physical Biochemistry

Section I

Biological Macromolecule: general principles, molecular interaction in macromolecular structure, the environment in the cell, symmetry relationships between molecules, the structure of proteins, the structure of nucleic acids.

Thermodynamic principles: First law of thermodynamics. Molecular interpretation of thermodynamic quantities, Second law of thermodynamics.

Section II

Molecular thermodynamics: Complexities in modeling macromolecular structure, Molecular mechanics, Stabilizing interactions in macromolecules, simulating macromolecular structure.

Section III

Methods for separation and characterization of macromolecule: General principles, sedimentation, Electrophoresis and isoelectric focusing, Mass spectrometry.

X-ray diffraction: Structures of atomic resolution, crystals, theory of x-ray diffraction, determining the crystal morphology, solving macromolecular structures by x-ray diffraction, fiber diffraction.

Section IV

Quantum mechanics and spectroscopy: Light and transitions, postulate approach to quantum mechanics, transition energies,

Nuclear magnetic resonance spectroscopy

Text Book:

VanHold KE, Johnson WC and HOPS(1998) Principles of Physical Biochemistry. Prentice Hall Inc., Upper Saddle River, New Jersey 07458